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[Seminar Notification] Dr. Kim, Sang-Heon_04.21.2016
2016-04-19

1. Title : Self-organized 3D microtissue for regenerative medicine and drug discovery

2. Speaker : Kim, Sang-Heon, Ph.D.

3. Affiliation : Principle Research Scientist/Professor, Center for Biomaterials, Biomedical Engineering Institute,
                       Korea Institute of Science and Technology (KIST) University of Science and Technology (UST)

4. Date : 04. 21. 2016 16:00-17:00

5. Place : SIMS Main Building, Auditorium 109 (1F)

6. Abstract

Over the past decade, the concept of tissue engineering has been extended to include technologies that use multi-cellular aggregates, not only to repair or replace tissue, but as a drug screening system with well-defined biological outputs. Within stem cell research as well as tissue engineering, synthetic biomimetic materials have been designed as artificial ECMs (art-ECMs) to stimulate cell adhesion and particular cellular functions and the resulting field has been termed “matrix engineering”. In our strategies, bioactive polypeptides, such as growth factor (GF) or cytokine, fused materials have been employed as art-ECMs to enhance selective interactions between materials and cells and to induce specific functions of the cells. In stem cell and tissue engineering research, studies involving cellular adhesion to an art-ECM have recently heightened. Recently, we demonstrated that heparin sulfate proteoglycan (HSPG) on cell membrane are involved in the cell adhesion via the heparin-binding region of fibronectin. In our previous study, FGF2-immobilized surface was synthesized as a HSPG targeting matrix for stem cell adhesion and was characterized based upon immobilization and biochemical and biological activities. A novel self-organized 3D microtissue, which was obtained by culturing mesenchymal cells on a substrate with the immobilized growth factor, showed various tissue functions such as angiogenesis, fibrosis, and skin-aging. We demonstrated the mechanism of 3D microtissue formation; HSPG-mediated adhesion of cells to FGF2-immobilized substrate is regulated by integrin signaling and leads to reduction in cell-matrix adhesive force. Here, we describe an innovative 3D microtissue culture based on cell adhesion (basic fibroblast growth factor [FGF2]-immobilized substrate) and assess the therapeutic/drug-screening potential of 3D microtissue composed of human mesenchymal cells such as adipose-derived stem cells and fibroblasts.